1064 .
GEOSPOROBACTER MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
7.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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KCl | 0.50 | g | ||
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KH2PO4 | 0.20 | g | ||
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NaCl | 1.00 | g | ||
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NH4Cl | 0.05 | g | ||
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CaCl2 x 2 H2O | 0.10 | g | ||
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MgCl2 x 6 H2O | 0.40 | g | ||
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Yeast extract | 1.00 | g | ||
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Glucose | 0.36 | g | ||
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Trace vitamins | 1.00 | ml | ||
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Trace element solution | 1.00 | ml | ||
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NaHCO3 | 0.84 | g | ||
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Na2S x 9 H2O | 0.50 | g | ||
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Resazurin | 1.00 | mg | ||
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Distilled water | 1000.00 | ml | ||
| 1 Mix ingredients except NaHCO3, Trace vitamins and Na2S x 9H2O, and autoclave under a N2-CO2 (90:10) atmosphere. Filter-sterilize NaHCO3 as an 8.4% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (90:10) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 7.5 - 8.0. | |||||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
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Folic acid | 2.0 | mg |
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Pyridoxine hydrochloride | 10.0 | mg |
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Thiamine HCl | 5.0 | mg |
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Riboflavin | 5.0 | mg |
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Nicotinic acid | 5.0 | mg |
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Calcium pantothenate | 5.0 | mg |
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Vitamin B12 | 0.1 | mg |
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p-Aminobenzoic acid | 5.0 | mg |
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Lipoic acid | 5.0 | mg |
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Distilled water | 1000.0 | ml |
Trace element solution #
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 0.640 | g | ||
|
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MgSO4 x 7 H2O | 6.200 | g | ||
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MnSO4 x n H2O | 0.550 | g | ||
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NaCl | 1.000 | g | ||
|
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FeSO4 x 7 H2O | 0.100 | g | ||
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CoCl2 x 6 H2O | 0.170 | g | ||
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CaCl2 x 2 H2O | 0.130 | g | ||
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ZnSO4 x 7 H2O | 0.180 | g | ||
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CuSO4 x 5 H2O | 0.050 | g | ||
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AlK(SO4)2 x 12 H2O | 0.018 | g | ||
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H3BO3 | 0.010 | g | ||
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Na2MoO4 x 2 H2O | 0.011 | g | ||
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NiCl2 x 6 H2O | 0.025 | g | ||
| 1 Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml. | |||||