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JCM 1084 . THIOBACILLUS PROSPERUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1084 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.33 g 0.33 4.427
MgCl2 x 6 H2O 2.75 g 2.75 13.527
MgSO4 x 7 H2O 3.45 g 3.45 13.997
NH4Cl 1.25 g 1.25 23.369
CaCl2 x 2 H2O 0.14 g 0.14 0.952
KH2PO4 0.14 g 0.14 1.029
K2HPO4 0.14 g 0.14 0.804
NaCl 0.50 g 0.5 8.556
NiCl2 x 6 H2O 2.00 mg 0.002 0.015
Trace mineral solution 10.00 ml - -
Distilled water 940.00 ml - -
1 Adjust pH to 2.5 and autoclave. After cooling, aseptically add the following solution (filter-sterilized):
FeSO4 x 7H2O solution 50.00 ml - -

Trace mineral solution #

1000
Solution occurs in 4 other media (first defined in Medium J1084).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace minerals 1000.00 ml - -
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 -

FeSO4 x 7H2O solution #

Equipment needed: Filter
1 Dissolve 40.0 g FeSO4 x 7H2O in 0.1 N H2SO4 solution and fill up to 100.0 ml. Filter-sterilize and store under a N2 atmosphere.

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 2.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 3.48 14.119
MgCl2 x 6 H2O 2.75 13.527
NH4Cl 1.25 23.369
NaCl 0.51 8.727
KCl 0.33 4.427
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain