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JCM 1085 . ANAEROBACTER MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1085 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.317 5.921
KH2PO4 0.33 g 0.317 2.327
KCl 0.33 g 0.317 4.248
CaCl2 x 2 H2O 0.33 g 0.317 2.154
MgCl2 x 6 H2O 0.33 g 0.317 1.558
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Yeast extract 1.00 g 0.96 -
Resazurin 1.00 mg 9.6e-4 0.004
Distilled water 1000.00 ml - -
1 Mix components thoroughly, adjust pH to 7.0 and autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following sterile solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere or *filter-sterilized and stored under 80% N2 and 20% CO2 gas mixture):
Sucrose
(w/v, 20%) 		10.00 ml - -
NaHCO3
(*, 8%) 		18.30 ml - -
2 Dispense the medium into culture vessels under a N2 atmosphere and seal with butyl rubber stoppers. Finally, add per litter the following solutions (autoclaved and stored under a N2 atmosphere):
L-Cysteine HCl x H2O
(5%) 		6.00 ml - -
Na2S x 9 H2O
(5%) 		6.00 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 0.96 -
NH4Cl 0.317 5.921
KCl 0.317 4.248
KH2PO4 0.317 2.327
CaCl2 x 2 H2O 0.317 2.154
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain