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JCM 1126 . SULFATE-FREE METAKO MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1126 #

ml
Equipment needed: Filter Autoclave Hungate tubes Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 9.47 g 9.303 45.757
CaCl2 x 2 H2O 1.36 g 1.336 9.087
NaCl 20.75 g 20.383 348.787
KCl 0.60 g 0.589 7.906
NH4Cl 0.54 g 0.53 9.917
KH2PO4 0.14 g 0.138 1.011
NaHCO3 2.69 g 2.642 31.455
KBr 85.00 mg 0.083 0.702
Na2B4O7 x 10 H2O 34.00 mg 0.033 -
SrCl2 x 6 H2O 12.00 mg 0.012 0.044
NaF 3.00 mg 0.003 0.07
LiCl 1.00 mg 9.8e-4 0.023
Trace element solution 1.00 ml - -
Selenite-tungstate solution 0.40 ml - -
Trace vitamins solution 2.00 ml - -
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 1000.00 ml - -
1 Mix components thoroughly and distribute the medium into culture vessels (e.g., 20 ml medium in 100 ml serum bottles or 5.0 ml medium in Hungate tubes) under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave. Allow to stand the autoclaved medium overnight. Add per liter the following solution from a sterile anaerobic stock (filter-sterilized and stored under a N2 atmosphere):
Trimethylamine
(1 M) 		10.00 ml - -
2 Prior to inoculation, add per liter the following solutions aseptically and anaerobically (autoclaved and stored under a N2 atmosphere):
L-Cysteine HCl x H2O
(5%) 		2.50 ml - -
Na2S x 9 H2O
(5%) 		2.50 ml - -

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 0.150 g 0.15 0.785
MnCl2 x 4 H2O 0.610 g 0.61 -
CoCl2 x 6 H2O 0.420 g 0.42 3.235
ZnCl2 0.090 g 0.09 0.66
CuCl2 x 2 H2O 7.000 mg 0.007 0.041
AlCl3 6.000 mg 0.006 0.045
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.020 g 0.02 0.083
SrCl2 x 6 H2O 0.010 g 0.01 0.038
NaBr 0.010 g 0.01 0.097
KI 0.070 g 0.07 0.27
FeCl3 x 6 H2O 0.500 g 0.5 1.85
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Distilled water 1000.000 ml - -
1 Comment: For strain JCM 32479, replace trimethylamine with sodium lactate (final 0.55 g/L) and yeast extract (1.0 g/L).

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Trace vitamins solution #

1000
Solution occurs in 3 other media (first defined in Medium J682).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.9 mg 0.005 0.02
Folic acid 8.8 mg 0.009 0.02
Pyridoxine hydrochloride 4.1 mg 0.004 -
Thiamine HCl 6.7 mg 0.007 -
Riboflavin 7.5 mg 0.008 0.02
Nicotinic acid 2.5 mg 0.003 0.02
DL-Calcium pantothenate 9.5 mg 0.01 0.02
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 2.7 mg 0.003 0.02
Lipoic acid 4.1 mg 0.004 0.02
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20.383 348.787
MgCl2 x 6 H2O 9.303 45.757
NaHCO3 2.642 31.455
CaCl2 x 2 H2O 1.336 9.087
KCl 0.589 7.906
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