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JCM 1148 . NITROSOSPHAERA GARGENSIS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1148 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.050 g 0.049 0.36
KCl 0.075 g 0.073 0.984
MgSO4 x 7 H2O 0.050 g 0.049 0.198
NaCl 0.584 g 0.571 9.778
CaCO3 4.000 g 3.914 39.104
Distilled water 1000.000 ml - -
1 Mix components and autoclave (CaCO3 will not fully dissolve). After cooling, add the following solutions to the medium (autoclaved or *filter-sterilized):
NH4Cl
(50 mM) 		20.000 ml - -
Trace element solution
(*) 		1.000 ml - -
Selenite-tungstate solution 0.500 ml - -
2 Comments: Typically, prepare the medium in glass bottles with plastic caps leaving an ample head space (e.g., 50 ml medium in 250 ml-Duran® glass bottles). Cultivate in the dark without agitation. Check growth by phase-contrast microscopy. Also, it can be checked by measuring ammonium concentration. Once ammonium in the medium is depleted, it can be fed by the addition of another 50 mM NH4Cl solution (final 1.0 mM). The ammonium-feeding can be repeated several times. If the culture stops the ammonium oxidation or begins to grow even slower, nitrite may be accumulated up to a level causing growth inhibition in the culture (ca. 15 mM). If so, replace the medium as follows: spin down the culture (< 65,000 g for 15 min), remove the supernatant, and add fresh culture medium to the white pellet. Then, suspend the pellet and remove the suspension to a new glass bottle.

Trace element solution #

1002
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(conc.) 		2.500 ml - -
MnSO4 x 5 H2O 0.048 g 0.048 0.199
H3BO3 0.050 g 0.05 0.807
ZnCl2 0.070 g 0.07 0.513
Na2MoO4 x 2 H2O 0.073 g 0.073 0.301
CuCl2 x 2 H2O 0.020 g 0.02 0.117
NiCl2 x 6 H2O 0.024 g 0.024 0.185
CoCl2 x 6 H2O 0.080 g 0.08 0.615
FeSO4 x 7 H2O 1.000 g 0.998 3.59
Distilled water 1000.000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Archaea
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
CaCO3 3.914 39.104
NaCl 0.571 9.778
KCl 0.073 0.984
KH2PO4 0.049 0.36
MgSO4 x 7 H2O 0.049 0.198
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
temperature thermophilic 2 strains
temperature mesophilic 1 strain