Name: MMFC1 MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J1167 #

Equipment needed: Autoclave Anaerobic workbench Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.227	g	0.112	0.822
K₂HPO₄	0.348	g	0.172	0.985
NH₄Cl	0.500	g	0.246	4.607
NaCl	2.250	g	1.109	18.975
MgSO₄ x 7 H₂O	0.500	g	0.246	1
CaCl₂ x 2 H₂O	0.025	g	0.012	0.084
Trace mineral solution (see below)	1.000	ml	-	-
Yeast extract	2.000	g	0.986	-
Resazurin	0.500	mg	2.5e-4	0.001
Distilled water	1000.000	ml	-	-
1 Prepare medium under a N₂ gas atmosphere. After autoclaving, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
Trace vitamins (*)	20.000	ml	-	-
Methanol (v/v, 50%)	8.000	ml	-	-
L-Cysteine HCl x H₂O (5%)	0.350	ml	-	-
2 Trace mineral solution:
Na₂-EDTA	22.590	mg	0.011	0.033
MgSO₄ x 7 H₂O	246.000	mg	0.121	0.492
CaCl₂ x 2 H₂O	14.000	mg	0.007	0.047
H₃BO₃	3.500	mg	0.002	0.028
NaCl	0.584	mg	2.9e-4	0.005
FeSO₄ x 7 H₂O	1.500	mg	7.4e-4	0.003
CoCl₂ x 6 H₂O	1.200	mg	5.9e-4	0.005
NiCl₂ x 6 H₂O	1.190	mg	5.9e-4	0.005
Na₂MoO₄ x 2 H₂O	0.944	mg	4.7e-4	0.002
Na₂SeO₃ x 5 H₂O	0.395	mg	1.9e-4	7.4e-4
MnCl₂ x 4 H₂O	0.257	mg	1.3e-4	-
ZnCl₂	0.136	mg	6.7e-5	4.9e-4
CuCl₂ x 2 H₂O	0.205	mg	1.0e-4	5.9e-4
Distilled water	1000.000	ml	-	-
3 Adjust final pH of the medium to 6.5, if necessary.

Trace vitamins #

1000

Solution occurs in 220 other media (first defined in Medium J197).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Biotin	2.0	mg	0.002	0.008
Folic acid	2.0	mg	0.002	0.005
Pyridoxine hydrochloride	10.0	mg	0.01	0.049
Thiamine HCl	5.0	mg	0.005	0.015
Riboflavin	5.0	mg	0.005	0.013
Nicotinic acid	5.0	mg	0.005	0.041
Calcium pantothenate	5.0	mg	0.005	0.01
Vitamin B₁₂	0.1	mg	1.0e-4	7.4e-5
p-Aminobenzoic acid	5.0	mg	0.005	0.036
Lipoic acid	5.0	mg	0.005	0.024
Distilled water	1000.0	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	6.5
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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