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JCM 1282 . HNN MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1282 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.00 g 24.753 423.554
MgCl2 x 6 H2O 4.20 g 4.158 20.454
MgSO4 x 7 H2O 3.40 g 3.366 13.658
KCl 0.50 g 0.495 6.641
NH4Cl 0.25 g 0.248 4.628
K2HPO4 0.14 g 0.139 0.796
CaCl2 x 2 H2O 0.70 g 0.693 4.714
NaHCO3 1.00 g 0.99 11.786
Trace mineral solution 10.00 ml - -
Distilled water 1000.00 ml - -
1 Mix components, except NaHCO3, and autoclave under N2 atmosphere. Filter-sterilize 8% (w/v) NaHCO3 solution and add to the medium. Aseptically distribute the medium into culture vessels and seal with butyl rubber stoppers. Replace gas phase with N2O, After inoculation, pressurize the inoculated bottles to 300 kPa H2-CO2 (4:1, v/v).

Trace mineral solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace minerals 1000.000 ml - -
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.100 g 0.1 0.303

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24.762 423.723
MgCl2 x 6 H2O 4.158 20.454
MgSO4 x 7 H2O 3.396 13.778
NaHCO3 0.99 11.786
CaCl2 x 2 H2O 0.694 4.721
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain