J1327
1327 .
GEOBACTER NBAF MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1327 #
ml
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.42 | g | 0.415 | 3.053 | |
| K2HPO4 | 0.22 | g | 0.218 | 1.249 | |
| NH4Cl | 0.20 | g | 0.198 | 3.698 | |
| KCl | 0.38 | g | 0.376 | 5.042 | |
| NaCl | 0.36 | g | 0.356 | 6.093 | |
| CaCl2 x 2 H2O | 0.04 | g | 0.04 | 0.269 | |
| Fe2(SO4)3 x n H2O | 0.01 | g | 0.01 | - | |
| MgSO4 x 7 H2O | 0.10 | g | 0.099 | 0.401 | |
|
Na2SeO4
(0.1%) |
1.00 | ml | - | - | |
| NB Mineral elixir solution | 10.00 | ml | - | - | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Mix components and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture, and add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized). | |||||
|
NaHCO3
(*, 8%) |
20.00 | ml | - | - | |
|
Na2CO3
(*, 5%) |
10.00 | ml | - | - | |
|
Trace vitamins
(*) |
15.00 | ml | - | - | |
|
Sodium acetate
(1 M) |
15.00 | ml | - | - | |
|
Sodium fumarate
(1 M) |
40.00 | ml | - | - | |
| 2 Check final pH to be around 7.0. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 2.140 | g | - | - | |
| MnCl2 x 4 H2O | 0.100 | g | - | - | |
| FeSO4 x 7 H2O | 0.300 | g | - | - | |
| CoCl2 x 6 H2O | 0.170 | vg | - | - | |
| ZnSO4 x 7 H2O | 0.200 | vg | - | - | |
| CuCl2 x 2 H2O | 0.030 | vg | - | - | |
| AlK(SO4)2 | 0.003 | vg | - | - | |
| H3BO3 | 5.000 | mg | - | - | |
| Na2MoO4 x 2 H2O | 0.090 | g | - | - | |
| NiSO4 x 6 H2O | 0.110 | g | - | - | |
| Na2WO2 x 2 H2O | 0.020 | g | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 8.0-8.5 with NaOH. Then proceed to add minerals. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 8.2 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |