J146
146 .
MODIFIED OTI MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J146 #
ml
Equipment needed: Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Polypeptone
(BD-BBL) |
5.00 | g | 5 | - | |
|
Heart Infusion Broth
(BD-Difco) |
5.00 | g | 5 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| Pectin | 0.80 | g | 0.8 | 4.121 | |
| Glucose | 0.80 | g | 0.8 | 4.441 | |
| Starch | 0.80 | g | 0.8 | - | |
| Sucrose | 0.80 | g | 0.8 | 2.337 | |
| Maltose | 0.80 | g | 0.8 | 2.337 | |
| Sodium pyruvate | 0.80 | g | 0.8 | 7.27 | |
| Ribose | 0.80 | g | 0.8 | 5.329 | |
| K2HPO4 | 2.00 | g | 2 | 11.483 | |
| NaCl | 5.00 | g | 5 | 85.558 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
| L-Cysteine HCl x H2O | 0.68 | g | 0.68 | - | |
| Clarified rumen fluid | 500.00 | ml | - | - | |
| Distilled water | 500.00 | ml | - | - | |
| Agar | 1.60 | g | 1.6 | 4.757 | |
| 1 Adjust pH to 7.0. Prepare medium anaerobically and dispense into rubber-stoppered tubes under oxygen-free N2. Add inactivated sterile rabbit serum to give final concentration in each tube of 5%. Add filter-sterilized thiamine pyrophosphate to each tube to give a final concentration of 0.75 mg/100 ml. Subculture with a Pasteur pipette (20-30 drops). Gas phase for culture system should be oxygen-free N2 or N2-CO2 (90:10). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Filter |
| Edit in Medium builder | |