J159
159 .
THERMOCOCCUS CELER MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Thermococcus celer DSM 2476
This strain uses the standard medium without modifications.
Main sol. J159 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Modified Brock's salt base solution | 1000.0 | ml | - | - | |
| NaCl | 40.0 | g | 40 | 684.463 | |
|
Yeast extract
(BD-Difco) |
2.0 | g | 2 | - | |
|
Sulfur
(powder) |
10.0 | g | 10 | 311.818 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| 1 Mix ingredients, except yeast extract, sulfur and Na2S x 9H2O, adjust pH to 5.8 with H2SO4, and autoclave under a N2 atmosphere. Separately autoclave yeast extract as a 10% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add the yeast extract solution and sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 5.8, if necessary. | |||||
Solution occurs in 13
other media (first defined in Medium J165).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.3 | 9.838 |
| KH2PO4 | 0.28 | g | 0.28 | 2.058 |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 |
| CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 |
| FeCl3 x 6 H2O | 2.00 | mg | 0.002 | 0.007 |
| MnCl2 x 4 H2O | 1.80 | mg | 0.002 | 0.009 |
| Na2B4O7 x 10 H2O | 4.50 | mg | 0.005 | - |
| ZnSO4 x 7 H2O | 0.22 | mg | 2.2e-4 | 7.7e-4 |
| CuCl2 x 2 H2O | 0.05 | mg | 5.0e-5 | 2.9e-4 |
| Na2MoO4 x 2 H2O | 0.03 | mg | 3.0e-5 | 1.2e-4 |
| VOSO4 x n H2O | 0.03 | mg | 3.0e-5 | - |
| CoSO4 x 7 H2O | 0.01 | mg | 1.0e-5 | 6.5e-5 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 5.8 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Thermococcus celer DSM 2476
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 40 | 684.463 |
| Sulfur | 10 | 311.818 |
| Yeast extract | 2 | - |
| (NH4)2SO4 | 1.3 | 9.838 |
| Na2S x 9 H2O | 0.5 | 2.082 |
| Show more | ||
