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J236. THERMOCLADIUM MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Modified Brock's salt base solution 1000.00 ml - -
2 2.94 g 2.911 -
3
(BD-Difco)
0.50 g 0.495 -
4 Trace vitamins 10.00 ml - -
5 1.00 mg 9.9e-4 0.004
6 0.50 g 0.495 2.061
7
(powder)
10.00 g 9.901 308.769
Instructions:
  1. Combine Modified Brock's salt base solution, trisodium citrate and resazurin, and adjust pH to 3.5 with 10 N H2SO4. Autoclave under a N2 atmosphere. Separately autoclave yeast extract and Na2S x 9H2O as 10% and 5% solutions, respectively, under a N2 atmosphere. Filter-sterilize Trace vitamins. Steam sulfur for 3 hr on each of 3 successive days. Aseptically mix components except Na2S x 9H2O in suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) while gassing with H2-CO2 (80:20) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, aseptically and anaerobically add the Na2S x 9H2O solutions and adjust pH to 4.0-4.5, if necessary. When the indicator ( resazurin) turns to colorless, inoculate the culture and pressurize the vessels to 100 kPa H2-CO2 (80:20).
Solution occurs in 11 other media (first defined in medium J165 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.30 g 1.3 9.838
2 0.28 g 0.28 2.058
3 0.25 g 0.25 1.014
4 0.07 g 0.07 0.476
5 2.00 mg 0.002 -
6 1.80 mg 0.002 -
7 4.50 mg 0.005 -
8 0.22 mg 2.2e-4 -
9 0.05 mg 5.0e-5 -
10 0.03 mg 3.0e-5 1.2e-4
11 0.03 mg 3.0e-5 -
12 0.01 mg 1.0e-5 -
13 1000.00 ml 998.207 55408.8
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 9

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Sulfur 9.901 308.769
Trisodium citrate x 2H2O 2.911 -
(NH4)2SO4 1.287 9.741
Na2S x 9H2O 0.495 2.061
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