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JCM 244 . JCM MEDIUM No. 244

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Medium for Thermococcus aegaeus DSM 12767

This strain uses the standard medium without modifications.

Main sol. J244 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Synthetic seawater (2 x)
(2 x) 		500.00 ml - -
NaHCO3 0.16 g 0.156 1.858
KH2PO4 0.50 g 0.488 3.585
Trace minerals 15.00 ml - -
Ni-Se-W solution 10.00 ml - -
Bacto peptone
(BD-Difco) 		2.00 g 1.951 -
Yeast extract
(BD-Difco) 		1.00 g 0.976 -
Resazurin 0.75 mg 7.3e-4 0.003
Na2S x 9 H2O 0.50 g 0.488 2.031
Sulfur
(powder) 		10.00 g 9.756 304.213
Distilled water 500.00 ml - -
1 Mix ingredients, except sulfur and Na2S x 9H2O, and adjust pH to 6.0 with H2SO4. Sterilize the medium by filtration. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Distribute the medium and sulfur into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 6.0 with sterile 1 N H2SO4. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).

Synthetic seawater (2 x) #

1000
Solution occurs in 4 other media (first defined in Medium J244).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 55.40 g 55.4 947.981
MgSO4 x 7 H2O 14.00 g 14 56.801
MgCl2 x 6 H2O 11.00 g 11 54.106
KCl 1.30 g 1.3 17.438
NaBr 0.20 g 0.2 1.944
H3BO3 0.06 g 0.06 0.97
SrCl2 x 6 H2O 0.03 g 0.03 0.113
KI 0.10 mg 1.0e-4 3.9e-4
Trisodium citrate 20.00 mg 0.02 0.077
CaCl2 x 2 H2O 1.50 g 1.5 10.203
Distilled water 1000.00 ml - -

Ni-Se-W solution #

1000
Solution occurs in 3 other media (first defined in Medium J244).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NiCl2 x 6 H2O 25.0 mg 0.025 0.193
(NH4)2Ni(SO4)2 x 6 H2O 2.0 g 2 5.063
Na2SeO3 0.3 mg 3.0e-4 -
Na2SeO4 10.0 mg 0.01 0.053
Na2WO4 x 2 H2O 10.0 mg 0.01 0.03
Distilled water 1000.0 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 3
Thermococcus aegaeus DSM 12767

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 27.039 462.68
Sulfur 9.756 304.213
MgSO4 x 7 H2O 6.873 27.886
MgCl2 x 6 H2O 5.366 26.393
Bacto peptone 1.951 -
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Cultivation metadata from 3 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 2 3 strains
oxygen anaerobe 3 strains
oxygen obligate anaerobe 1 strain
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