Name: MJTSO MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J446 #

Equipment needed: Autoclave Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	3.00	g	2.97	50.826
MgCl₂ x 6 H₂O	0.34	g	0.337	1.656
MgSO₄ x 7 H₂O	0.42	g	0.416	1.687
KCl	0.05	g	0.05	0.664
K₂HPO₄	0.14	g	0.139	0.796
NH₄Cl	0.25	g	0.248	4.628
CaCl₂ x 2 H₂O	0.07	g	0.069	0.471
Trace mineral solution	10.00	ml	-	-
Na₂S₂O₃ x 5 H₂O	1.00	g	0.99	3.989
Sulfur (powder)	3.00	g	2.97	92.619
NaHCO₃	1.00	g	0.99	11.786
Distilled water	1000.00	ml	-	-
1 Mix ingredients except NaHCO₃ and sulfur, adjust pH to 7.5 with NaOH and autoclave. Filter-sterilize 8% NaHCO₃ solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.06 g sulfur) under a stream of CO₂-O₂ (93:7, v/v) gas mixture, seal with butyl rubber stoppers and readjust pH to 7.5 with NaOH. Pressurize the inoculated vessel to 100 kPa CO₂-O₂ (93:7, v/v).

Trace mineral solution #

1000

Solution occurs in 1 other media (first defined in Medium J446).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂-EDTA	0.50	g	0.5	-
CoCl₂ x 6 H₂O	0.15	g	0.15	1.155
MnCl₂ x 4 H₂O	0.10	g	0.1	-
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
ZnCl₂	0.10	g	0.1	0.734
AlCl3 x 6 H₂O	0.04	g	0.04	0.166
Na₂WO₂ x 2 H₂O	0.03	g	0.03	-
CuCl₂ x 2 H₂O	0.02	g	0.02	0.117
NiSO₄ x 6 H₂O	0.02	g	0.02	0.129
H₂SeO₃	0.01	g	0.01	-
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-

All strains for this medium:

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phyla	1	1 strain
temperature	thermophilic	1 strain