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J508. MODIFIED MMJS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.00 g 19.782 338.493
2 0.09 g 0.089 0.511
3 0.07 g 0.069 0.509
4 0.80 g 0.791 5.383
5 0.25 g 0.247 4.623
6 1.00 g 0.989 11.637
7 4.00 g 3.956 16.052
8 3.00 g 2.967 14.596
9 0.33 g 0.326 4.378
10 1.00 mg 9.9e-4 -
11 1.00 mg 9.9e-4 -
12 0.01 g 0.01 -
13 1.00 mg 9.9e-4 0.004
14 2.00 g 1.978 -
15
(powder)
3.00 g 2.967 92.539
16 Trace mineral solution 10.00 ml - -
17 Trace vitamins 1.00 ml - -
18 2.00 g 1.978 23.549
19 1000.00 ml 987.346 54806
Instructions:
  1. Mix ingredients except sulfur, Trace vitamins and NaHCO3, adjust pH to 6.8 and autoclave. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sulfur under a stream of N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Replace the gas phase with N2-CO2-O2 (79:20:1, v/v) and pressurized inoculated bottles to 200 kPa with the same gas mixture.
Solution occurs in 1 other media (first defined in medium J508 )
Instructions:
  1. Use Trace mineral solution of Medium No. 413 with 0.01 g/L (final) Na2MoO4 x 2H2O.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 988.333 54860.8
NaCl 19.782 338.493
MgSO4 x 7H2O 3.956 16.052
Sulfur 2.967 92.539
MgCl2 x 6H2O 2.967 14.596
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