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JCM 556 . METHYLOSOMA DIFFICILE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J556 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 0.100 g 0.1 1.708
MgCl2 x 6 H2O 0.040 g 0.04 0.196
KCl 0.050 g 0.05 0.669
CaCl2 x 2 H2O 0.015 g 0.015 0.102
Na2SO4 0.016 g 0.016 0.112
Distilled water 993.000 ml - -
1 Mix components and autoclave. After cooling, add the following solutions (autoclaved or filter-sterilized) to the medium:
HEPES buffer
(pH 7.0, 2 M) 		5.000 ml - -
1 M Phosphate buffer
(pH 7.2, 1 M) 		0.150 ml - -
KNO3
(0.09 M) 		0.544 ml - -
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
Vitamin solution
(filter-sterilized) 		1.000 ml - -
2 Distribute the culture medium into culture tubes (leave >80% volume as a gas phase) and seal with butyl rubber stoppers.
3 Finally, replace the gas phase with 20% methane and 5% CO2 in air (by volume).

1 M Phosphate buffer #

Solution occurs in 2 other media (first defined in Medium J556).
1 Mix 28 ml of 1 M KH2PO4 and 78 ml of 1 M Na2HPO4 to prepare 100 ml of 1 M phosphate buffer.

Vitamin solution #

1000
Solution occurs in 4 other media (first defined in Medium J556).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
p-Aminobenzoic acid 50 mg 0.05 0.365
Biotin 10 mg 0.01 0.041
Nicotinic acid 100 mg 0.1 0.812
DL-Calcium pantothenate 25 mg 0.025 0.052
Pyridoxine hydrochloride 250 mg 0.25 -
Thiamine HCl 50 mg 0.05 -
Distilled water 1000 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Gas composition

  • 5 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.1 1.708
KCl 0.05 0.669
MgCl2 x 6 H2O 0.04 0.196
Na2SO4 0.016 0.112
CaCl2 x 2 H2O 0.015 0.102
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Cultivation metadata from 2 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
oxygen microaerophile 1 strain
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