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JCM 558 . MAGNETOSPIRILLUM MEDIUM (A)

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
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Medium for Magnetospirillum JCM 12779

This strain uses the standard medium without modifications.

Main sol. J558 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 1.60 g 1.581 11.137
KH2PO4 1.00 g 0.988 7.261
NH4Cl 0.50 g 0.494 9.237
K2SO4 0.06 g 0.059 0.34
KNO3 0.51 g 0.504 4.985
Trace x 10 solution 0.10 ml - -
Se/W solution 1.00 ml - -
Mg/Ca solution 5.00 ml - -
Na benzoate solution 72.0 g/L 2.00 ml - -
7 Vitamins solution 0.50 ml - -
FeSO4 solution (5 g/L) with EDTA 3.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 7.2 - 7.4.
2 Separately autoclave Trace x 10 solution, Se/W solution and Mg/Ca solution, and filter-sterilize Na benzoate solution, 7 vitamins solution and FeSO4 solution. Aseptically add all solutions to the medium.

Trace x 10 solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 6 mg 0.06 0.97
CuCl2 x 2 H2O 2 mg 0.02 0.117
Distilled water 100 ml - -

Se/W solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.05 g 0.5 12.501
Na2SeO3 0.20 mg 0.002 -
Na2WO4 x 2 H2O 0.40 mg 0.004 0.012
Distilled water 100.00 ml - -

Mg/Ca solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.0 g 20 98.375
CaCl2 x 2 H2O 0.5 g 5 34.01
Distilled water 100.0 ml - -

Na benzoate solution 72.0 g/L #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium benzoate 7.2 g 72 499.653
Distilled water 100.0 ml - -

7 Vitamins solution #

100
Solution occurs in 1 other media (first defined in Medium J558).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 4 mg 0.04 0.03
D-Biotin 2 mg 0.02 0.082
D-Calcium pantothenate 5 mg 0.05 -
Thiamine HCl 10 mg 0.1 -
p-Aminobenzoic acid 10 mg 0.1 0.729
Nicotinic acid 20 mg 0.2 1.625
Pyridoxine hydrochloride 50 mg 0.5 -
Distilled water 100 ml - -

FeSO4 solution (5 g/L) with EDTA #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 5 -
FeSO4 x 7 H2O 0.05 g 0.5 1.798
Distilled water 100.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 5
Magnetospirillum JCM 12779

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 1.581 11.137
KH2PO4 0.988 7.261
KNO3 0.504 4.985
NH4Cl 0.494 9.237
Sodium benzoate 0.142 0.987
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Cultivation metadata from 5 strains BacDive

phyla 1 5 strains
temperature mesophilic 5 strains