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JCM 602 . DESULFOVIBRIO MAGNUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Solution A #

902
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 0.53 g 0.588 4.139
KH2PO4 0.41 g 0.455 3.34
NH4Cl 0.30 g 0.333 6.218
NaCl 0.30 g 0.333 5.691
Na2SO4 1.42 g 1.574 11.083
Sodium lactate 2.24 g 2.483 22.161
Acid trace element solution 1.00 ml - -
Alkaline trace element solution 1.00 ml - -
Distilled water 900.00 ml - -
1 Mix components and autoclave under a N2 gas atmosphere.

Acid trace element solution #

1010
Solution occurs in 1 other media (first defined in Medium J602).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(6 N) 		10.00 ml - -
H3BO3 61.80 mg 0.061 0.989
MnCl2 x 4 H2O 61.25 mg 0.061 -
FeCl2 x 4 H2O 943.50 mg 0.934 4.699
CoCl2 x 6 H2O 64.50 mg 0.064 0.492
NiCl2 x 6 H2O 12.86 mg 0.013 0.098
ZnCl2 67.70 mg 0.067 0.492
Distilled water 1000.00 ml - -

Alkaline trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium J602).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 400.0 mg 0.4 10.001
Na2SeO3 x 5 H2O 17.3 mg 0.017 0.066
Na2WO4 x 2 H2O 29.4 mg 0.029 0.089
Na2MoO4 x 2 H2O 20.5 mg 0.021 0.085
Distilled water 1000.0 ml - -

Solution B #

50
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.11 g 2.2 14.965
MgCl2 x 6 H2O 0.10 g 2 9.838
Distilled water 50.00 ml - -
1 Mix components and autoclave under a N2 atmosphere.

Solution C #

50
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2 g 40 476.151
Distilled water 50 ml - -
1 Mix components, filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture.
2 To complete the medium, combine Solutions A, B, C and 1 ml Vitamin solution (see below) aseptically under a stream of a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into sterilized culture vessels under the same gas mixture and seal with butyl rubber stoppers. Then, add 0.01 volume of 5% Na2S x 9H2O solution (autoclaved under a N2 atmosphere). Check the final pH to be 7.0 - 7.2. Pressurize the inoculated vessels to 70 kPa N2-CO2 (4:1, v/v) overpressure.

Vitamin solution #

1000
Solution occurs in 2 other media (first defined in Medium J602).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
p-Aminobenzoic acid 100 mg 0.1 0.729
Thiamine HCl 200 mg 0.2 -
DL-Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 500 mg 0.5 -
Vitamin B12 100 mg 0.1 0.074
Riboflavin 100 mg 0.1 0.266
Distilled water 1000 ml - -
1 Filter-sterilize and store under a N2 atmosphere.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.1
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Cultivation metadata from 1 strains BacDive

pH 2 - 4 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
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