J634
634 .
ALKALIPHILUS SUCCINACIDUS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J634 #
ml
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| K2HPO4 | 0.5 | g | 0.5 | 2.871 | |
| (NH4)2SO4 | 1.0 | g | 1 | 7.568 | |
| NaCl | 1.0 | g | 1 | 17.112 | |
| MgSO4 x 7 H2O | 0.1 | g | 0.1 | 0.406 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| Sodium succinate | 5.0 | g | 5 | 30.855 | |
| Yeast extract | 1.0 | g | 1 | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | - | |
| Na2S x 9 H2O | 0.1 | g | 0.1 | 0.416 | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components except L-cysteine x HCl x H2O and Na2S x 9H2O and adjust pH to 7.2. Bring to a boil for 5-10 sec and cool down under a stream of N2-CO2 (80:20, v/v). Distribute into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Separately autoclave L-cysteine x HCl x H2O and Na2S x 9H2O as 5% solutions under a N2 atmosphere. Prior to inoculation, add L-cysteine x HCl x H2O and Na2S x 9H2O solutions anaerobically. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |