J700
700 .
MJY MEDIUM FOR PYROLINEA MARINUS
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J700 #
ml
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MJ(-N) synthetic seawater | 1000.00 | ml | - | - | |
| NH4Cl | 0.25 | g | 0.246 | 4.605 | |
| NaNO3 | 0.50 | g | 0.493 | 5.796 | |
| Yeast extract | 1.00 | g | 0.985 | - | |
| 1 Mix components thoroughly. Distribute the medium into culture vessels (e.g., 20 ml medium in 50 ml serum bottles) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (*filter-sterilized or autoclaved, and stored anaerobically) to the medium: | |||||
|
NaHCO3
(*, 8.0%) |
5.00 | ml | - | - | |
|
Na2S x 9 H2O
(5.0%) |
10.00 | ml | - | - | |
| 2 Pressurize the culture vessels to 200 kPa N2-CO2 (4:1, v/v) . Check final pH to be 6.0-6.5. | |||||
| 3 Comment: MJ(-N) synthetic seawater can be replaced with commercially available synthetic seawater such as Jamarin S (Jamarin Laboratory). Addition of rumen fluid clarified (10.0 ml/L, see Medium No. 266) may stimulate growth. For strain JCM 15506, incubate with a 5-10% inoculum. | |||||
Solution occurs in 12
other media (first defined in Medium J268).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 30.00 | g | 29.703 | 508.264 |
| K2HPO4 | 0.14 | g | 0.139 | 0.796 |
| CaCl2 x 2 H2O | 0.14 | g | 0.139 | 0.943 |
| MgSO4 x 7 H2O | 3.40 | g | 3.366 | 13.658 |
| MgCl2 x 6 H2O | 4.18 | g | 4.139 | 20.357 |
| KCl | 0.33 | g | 0.327 | 4.383 |
| NiCl2 x 6 H2O | 0.50 | mg | 5.0e-4 | 0.004 |
| Na2SeO3 x 5 H2O | 0.50 | mg | 5.0e-4 | 0.002 |
| Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g | 0.01 | - |
| Modified Wolin's mineral solution | 10.00 | ml | - | - |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 6.2 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |