Name: SPOROSALIBACTERIUM MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J706 #

Equipment needed: Anaerobic workbench Filter Hungate tubes Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.30	g	0.319	2.343
K₂HPO₄	0.30	g	0.319	1.83
NH₄Cl	1.00	g	1.063	19.867
NaCl	40.00	g	42.508	727.378
CaCl₂ x 2 H₂O	0.20	g	0.213	1.446
Yeast extract	2.00	g	2.125	-
Trypticase peptone (BD-BBL)	2.00	g	2.125	-
Glucose	3.60	g	3.826	21.235
Trace element solution	1.00	ml	-	-
Resazurin	1.00	mg	0.001	0.005
Distilled water	940.00	ml	-	-
1 To complete the medium, add the following stock solutions [Filter-sterilize NaHCO₃ solution and store under a N₂-CO₂ (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N₂ atmosphere] to the medium (per 4.7 ml of the medium):
MgCl₂ x 6 H₂O (15%)	0.10	ml	-	-
NaHCO₃ (5%)	0.20	ml	-	-
L-Cysteine HCl x H₂O (5%)	0.05	ml	-	-
Na₂S x 9 H₂O (5%)	0.04	ml	-	-
2 Mix ingredients and adjust pH to 7.2. Distribute the medium into culture vessels (e.g., 4.7 ml of the medium in the Hungate tubes) under a N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.

Trace element solution #

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.2
Equipment needed:	Anaerobic workbench, Autoclave, Filter, Hungate tubes

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