JCM 706 . SPOROSALIBACTERIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Filter Hungate tubes Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.30 g 0.319 2.343
K2HPO4 0.30 g 0.319 1.83
NH4Cl 1.00 g 1.063 19.867
NaCl 40.00 g 42.508 727.378
CaCl2 x 2 H2O 0.20 g 0.213 1.446
Yeast extract 2.00 g 2.125 -
Trypticase peptone
(BD-BBL)
2.00 g 2.125 -
Glucose 3.60 g 3.826 21.235
Trace element solution 1.00 ml - -
Resazurin 1.00 mg 0.001 0.005
Distilled water 940.00 ml - -
1 To complete the medium, add the following stock solutions [Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere] to the medium (per 4.7 ml of the medium):
MgCl2 x 6 H2O
(15%)
0.10 ml - -
NaHCO3
(5%)
0.20 ml - -
L-Cysteine HCl x H2O
(5%)
0.05 ml - -
Na2S x 9 H2O
(5%)
0.04 ml - -
2 Mix ingredients and adjust pH to 7.2. Distribute the medium into culture vessels (e.g., 4.7 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 42.508 727.378
Glucose 3.826 21.235
Trypticase peptone 2.125 -
Yeast extract 2.125 -
NH4Cl 1.063 19.867
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain
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