J758
758 .
DETHIOSULFOVIBRIO II MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J758 #
ml
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.0 | g | 19.342 | 330.978 | |
| MgCl2 x 6 H2O | 3.0 | g | 2.901 | 14.271 | |
| KH2PO4 | 1.0 | g | 0.967 | 7.107 | |
| Trisodium citrate x H2O | 3.0 | g | 2.901 | - | |
|
Yeast extract
(BD-Difco) |
5.0 | g | 4.836 | - | |
|
Bacto peptone
(BD-Difco) |
2.0 | g | 1.934 | - | |
| FeCl2 solution | 1.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
| Resazurin | 0.5 | mg | 4.8e-4 | 0.002 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components and autoclave under a N2 atmosphere. After cooling, add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized and stored under a N2 atmosphere): | |||||
|
CaCl2 x 2 H2O
(10%) |
2.0 | ml | - | - | |
|
Na2S2O3 x 5 H2O
(*, 12.5%) |
20.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.0 | ml | - | - | |
| 2 Adjust pH of the medium to 6.7 - 6.8, if necessary. | |||||
Solution occurs in 87
other media (first defined in Medium J187).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(7.7 M, 25%) |
10.0 | ml | 2.5 | 68.568 |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
| Distilled water | 990.0 | ml | - | - |
Solution occurs in 89
other media (first defined in Medium J187).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnCl2 | 70 | mg | 0.07 | 0.514 |
| MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 |
| H3BO3 | 6 | mg | 0.006 | 0.097 |
| CoCl2 x 6 H2O | 190 | mg | 0.19 | 1.463 |
| CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 |
| NiCl2 x 6 H2O | 24 | mg | 0.024 | 0.185 |
| Na2MoO4 x 2 H2O | 36 | mg | 0.036 | 0.149 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 19.342 | 330.978 |
| Yeast extract | 4.836 | - |
| MgCl2 x 6 H2O | 2.901 | 14.271 |
| Trisodium citrate x H2O | 2.901 | - |
| Bacto peptone | 1.934 | - |
| Show more | ||
