J883
883 .
PETROTOGA AR80 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J883 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 10.00 | g | 9.891 | 169.254 | |
| MgSO4 x 7 H2O | 7.00 | g | 6.924 | 28.091 | |
| KCl | 0.34 | g | 0.336 | 4.511 | |
| NH4Cl | 0.25 | g | 0.247 | 4.623 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.138 | 0.942 | |
| KH2PO4 | 0.14 | g | 0.138 | 1.018 | |
|
Yeast extract
(BD-Difco) |
0.10 | g | 0.099 | - | |
| Trace mineral solution | 1.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| Resazurin | 0.20 | mg | 2.0e-4 | 8.6e-4 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.495 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.5 - 8.0. Bring to a boil and cool down under a N2 atmosphere. Distribute the medium into culture vessels under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| MgSO4 x 7 H2O | 3.0 | g | 3 | 12.172 |
| NaCl | 1.0 | g | 1 | 17.112 |
| MgSO4 x 7 H2O | 644.0 | mg | 0.644 | 2.613 |
| ZnSO4 x 7 H2O | 178.1 | mg | 0.178 | 0.619 |
| FeSO4 x 7 H2O | 100.0 | mg | 0.1 | 0.36 |
| CoCl2 x 6 H2O | 100.0 | mg | 0.1 | 0.77 |
| CaCl2 x 2 H2O | 75.5 | mg | 0.076 | 0.514 |
| CuSO4 x 5 H2O | 10.0 | mg | 0.01 | 0.04 |
| AlK(SO4)2 | 10.0 | mg | 0.01 | 0.039 |
| H3BO3 | 10.0 | mg | 0.01 | 0.162 |
| Na2MoO4 x 2 H2O | 10.0 | mg | 0.01 | 0.041 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |