MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 908 . HALORHODOSPIRA NEUTRIPHILA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J908 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 0.956 7.025
NH4Cl 0.50 g 0.478 8.936
NaCl 100.00 g 95.602 1635.91
MgCl2 x 6 H2O 2.00 g 1.912 9.405
MgSO4 x 7 H2O 0.50 g 0.478 1.939
CaCl2 x 2 H2O 0.05 g 0.048 0.325
Trace element solution SL-12 1.00 ml - -
NaHCO3
(10%, w/v) 		20.00 ml - -
Na2S x 9 H2O
(10%, w/v) 		7.50 ml - -
Seven vitamins solution 1.00 ml - -
Sodium acetate
(0.5 M) 		4.00 ml - -
Sodium succinate
(0.5 M) 		2.00 ml - -
Yeast extract
(5%) 		10.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0 with 1 M Na2CO3 or 1 M H2SO4. Mix ingredients, except NaHCO3, Na2S x 9H2O, seven vitamins solution, acetate solution, succinate solution and yeast extract solution, and autoclave under a N2-CO2 (80:20, v/v) atmosphere. Separately autoclave acetate solution, succinate solution and yeast extract solution. Filter-sterilize NaHCO3, Na2S x 9H2O. Aseptically and anaerobically add NaHCO3, Na2S x 9H2O, seven vitamins solution, acetate solution, succinate solution and yeast extract solution to the medium.

Trace element solution SL-12 #

1000
Solution occurs in 3 other media (first defined in Medium J496).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 3.0 g 3 -
FeSO4 x 7 H2O 1.1 g 1.1 3.957
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
MnCl2 x 4 H2O 50.0 mg 0.05 -
ZnCl2 42.0 mg 0.042 0.308
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 18.0 mg 0.018 0.074
H3BO3 300.0 mg 0.3 4.851
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
Distilled water 1000.0 ml - -
1 Autoclave separately.
2 Adjust pH to 6.8.

Seven vitamins solution #

200
Solution occurs in 1 other media (first defined in Medium J699).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 10 mg 0.05 0.037
p-Aminobenzoic acid 10 mg 0.05 0.365
D-(+)-biotin 2 mg 0.01 0.041
Nicotinic acid 20 mg 0.1 0.812
Calcium pantothenate 5 mg 0.025 0.052
Pyridoxine hydrochloride 50 mg 0.25 -
Thiamine-HCl x 2 H2O 10 mg 0.05 -
Distilled water 200 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 95.602 1635.91
MgCl2 x 6 H2O 1.912 9.405
KH2PO4 0.956 7.025
NH4Cl 0.478 8.936
MgSO4 x 7 H2O 0.478 1.939
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain