Name: HALANAEROBIUM SEHLINENSE MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J929 #

Equipment needed: Autoclave Hungate tubes Anaerobic workbench Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.3	g	0.278	2.045
K₂HPO₄	0.3	g	0.278	1.598
NH₄Cl	1.0	g	0.928	17.342
NaCl	200.0	g	185.529	3174.69
KCl	0.1	g	0.093	1.244
CaCl₂ x 2 H₂O	0.1	g	0.093	0.631
KNO₃	2.0	g	1.855	18.351
Trace minerals	10.0	ml	-	-
Yeast extract	1.0	g	0.928	-
Distilled water	1000.0	ml	-	-
1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 8.3 with 10 M KOH. Bring to a boil and cool down under a N₂ gas stream. Then, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave at 110C for 20 min. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) into the medium (per liter):
NaHCO₃ (*, 8%)	25.0	ml	-	-
MgCl₂ x 6 H₂O (10%)	15.0	ml	-	-
Glucose (1 M)	20.0	ml	-	-
Na₂S x 9 H₂O (5%)	8.0	ml	-	-

Trace minerals #

1000

Solution occurs in 97 other media (first defined in Medium J151).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x n H₂O	0.50	g	0.5	-
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.10	g	0.1	0.645
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂	0.01	g	0.01	0.039
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
Distilled water	1000.00	ml	-	-
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	8.3
Equipment needed:	Anaerobic workbench, Autoclave, Filter, Hungate tubes

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