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J945. MB MEDIUM FOR METHANOBACTERIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.15 g 0.148 1.089
2 0.30 g 0.296 1.702
3 0.60 g 0.593 10.145
4 0.30 g 0.296 2.243
5 0.12 g 0.119 0.481
6 0.08 g 0.079 0.538
7 Trace minerals solution 10.00 ml - -
8 1.00 mg 9.9e-4 0.004
9 932.00 ml 919.297 51028.7
10
(8%)
48.00 ml 8.192 97.519
11 Trace vitamins 10.00 ml - -
12
(5%)
6.00 ml - -
13
(5%)
6.00 ml - -
Instructions:
  1. Mix components thoroughly, and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (filter-sterilized): #10-11
  2. Distribute the medium into culture vessels (leave >80% gas phase by volume) under a H2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers. Finally, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere): #12-13
  3. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20, v/v).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 0.10 g 0.1 0.36
5 0.10 g 0.1 -
6 0.10 g 0.1 -
7 0.01 g 0.01 -
8 0.01 g 0.01 0.162
9 0.01 g 0.01 0.041
10 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 939.024 52123.7
NaHCO3 8.192 97.519
NaCl 0.593 10.145
(NH4)2SO4 0.296 2.243
K2HPO4 0.296 1.702
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