J951
951 .
C41 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J951 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.00 | g | 0.999 | 7.341 | |
| K2HPO4 | 0.50 | g | 0.5 | 2.868 | |
| Urea | 1.00 | g | 0.999 | 16.635 | |
| MgCl2 x 6 H2O | 0.50 | g | 0.5 | 2.457 | |
| CaCl2 x 2 H2O | 5.00 | mg | 0.005 | 0.034 | |
| FeSO4 x 7 H2O | 1.25 | mg | 0.001 | 0.004 | |
| L-Cysteine HCl x H2O | 0.60 | g | 0.599 | - | |
|
Yeast extract
(BD-Difco) |
2.00 | g | 1.998 | - | |
|
Tryptone
(BD-Difco) |
2.00 | g | 1.998 | 28.109 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
Solution occurs in 20
other media (first defined in Medium J433).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%, 7.7 M) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Edit in Medium builder | |