MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 954 . NEUTRAL OLIGOTROPHIC HALOARCHAEAL MEDIUM NO.3

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J954 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
MDS salt water 767.00 ml - -
Yeast extract 1.00 g 0.993 -
Fish peptone
(Fluka) 		0.25 g 0.248 -
Sodium formate 0.25 g 0.248 3.651
Sodium acetate 0.25 g 0.248 3.026
Sodium lactate 0.25 g 0.248 2.215
Sodium pyruvate 0.25 g 0.248 2.256
Distilled water 233.00 ml - -
1 Mix components thoroughly and adjust pH to 7.0. For preparation of solid medium, add 20.0 g/L agar. (if necessary, use washed agar as described in the recipe of Medium No. 574). Autoclave and, after cooling, add the following solutions (*filter-sterilized or autoclaved) to the medium.
NH4Cl
(*, 1 M) 		5.00 ml 0.267 5
Potassium phosphate buffer 2.00 ml - -

MDS salt water #

1000
Solution occurs in 5 other media (first defined in Medium J574).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240 g 240 4106.78
MgCl2 x 6 H2O 30 g 30 147.563
MgSO4 x 7 H2O 35 g 35 142.002
KCl 7 g 7 93.897
CaCl2
(1 M) 		5 ml 0.555 5
1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base.

Potassium phosphate buffer #

100
Solution occurs in 2 other media (first defined in Medium J574).
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4
(1 M) 		83.4 ml 14.526 83.4
KH2PO4
(1 M) 		16.6 ml 2.259 16.6
1 Combine the two solutions and check that pH is close to 7.5. Add an equal volume of distilled water.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 182.8 3128
MgSO4 x 7 H2O 26.658 108.158
MgCl2 x 6 H2O 22.85 112.394
KCl 5.332 71.518
Yeast extract 0.993 -
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

pH 6 - 8 2 strains
phyla 1 2 strains
oxygen aerobe 2 strains
temperature mesophilic 2 strains
temperature thermophilic 2 strains