Name: SEA SALTS TYG MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J958 #

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Sea Salt (Sigma)	50.0	g	49.31	-
Wolfe's mineral elixir	2.0	ml	-	-
Trypticase peptone (BD-BBL)	1.0	g	0.986	-
Yeast extract	1.0	g	0.986	-
Glucose	1.0	g	0.986	5.474
Resazurin	0.5	mg	4.9e-4	0.002
Na₂CO₃	1.0	g	0.986	9.305
Distilled water	1000.0	ml	-	-
1 Mix components thoroughly and autoclave under a N₂-CO₂ gas mixture. After cooling, adjust pH to 7.2 - 7.5 with 5% Na₂CO₃ solution (autoclaved and stored under N₂). Then, add per litter the following solutions (autoclaved and stored under N₂):
Na₂S x 9 H₂O (5%)	6.0	ml	-	-
L-Cysteine HCl x H₂O (5%)	6.0	ml	-	-

Wolfe's mineral elixir #

1000

Solution occurs in 8 other media (first defined in Medium J470).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
MgSO₄ x 7 H₂O	30.00	g	30	121.716
MnSO₄ x n H₂O	5.00	g	5	-
NaCl	10.00	g	10	171.116
FeSO₄ x 7 H₂O	1.00	g	1	3.597
CoCl₂ x 6 H₂O	1.80	g	1.8	13.863
CaCl₂ x 2 H₂O	1.00	g	1	6.802
ZnSO₄ x 7 H₂O	1.80	g	1.8	6.26
CuSO₄ x 5 H₂O	0.10	g	0.1	0.401
AlK(SO₄)₂ x 12 H₂O	0.18	g	0.18	-
H₃BO₃	0.10	g	0.1	1.617
Na₂MoO₄ x 2 H₂O	0.10	g	0.1	0.413
(NH₄)₂Ni(SO₄)₂ x 6 H₂O	2.80	g	2.8	7.089
Na₂WO₄ x 2 H₂O	0.10	g	0.1	0.303
Na₂SeO₄	0.10	g	0.1	0.529
Distilled water	1000.00	ml	-	-
1 First adjust pH to 1.0 with diluted H₂SO₄, then dissolve the salts.

All strains for this medium:

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