PUBLIC P2
P2 .
Taiyang Medium No.9 (prototype)
User-provided medium: This medium was provided by one of our users. Please be aware that we do not curate or test the media that are composed by our users. Be cautious when trying this medium, especially since no valid reference is given.
Culture medium recipe
Vol.
×
Main solution #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Clarified rumen fluid | 25.0 | ml/l | - | - | |
| Sheep blood | 25.0 | ml/l | - | - | |
| Artifical Sea Water | 50.0 | ml | - | - | |
| Peptone | 1.0 | g/l | 1 | - | |
| Yeast extract | 1.0 | g/l | 1 | - | |
| NaCl | 1.0 | g/l | 1 | 17.112 | |
| Cellulose | 0.5 | g/l | 0.5 | - | |
| Pectin | 0.5 | g/l | 0.5 | 2.575 | |
| Starch | 0.5 | g/l | 0.5 | - | |
| Inulin | 0.5 | g/l | 0.5 | - | |
|
Trehalose
(filtrate sterilization) |
0.5 | g/l | 0.5 | 1.461 | |
|
Sodium pyruvate
(filtrate sterilization) |
1.0 | g/l | 1 | 9.088 | |
| dextrin | 0.5 | g/l | 0.5 | - | |
|
L-Cysteine HCl
(filtrate sterilization) |
0.1 | g/l | 0.1 | 0.634 | |
|
Haemin solution
(filtrate sterilization) |
10.0 | ml | - | - | |
| agar (for solid plate medium) | 15.0 | g/l | 15 | - | |
| 1 Add all components, except trehalose/sodium pyruvate/L-Cys/sheep blood and haemin solution, autocleave 121℃ for 15min, add other components after autocleave, put the medium/agar in anaerobic workstation before use | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Haemin | 50 | mg | 0.5 | 0.767 | |
|
NaOH
(1 N) |
1 | ml | - | - | |
| Distilled water | 100 | ml | - | - | |
| 1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 23.47 | g/l | 23.47 | 401.608 | |
| Na2SO4 | 3.92 | g/l | 3.92 | 27.598 | |
| MgCl2 x 6 H2O | 10.64 | g/l | 10.64 | 52.336 | |
| CaCl2 | 1.10 | g/l | 1.1 | 9.912 | |
| NaHCO3 | 192.00 | mg/l | 0.192 | 2.286 | |
| KCl | 664.00 | mg/l | 0.664 | 8.907 | |
| KBr | 96.00 | mg/l | 0.096 | 0.807 | |
| H3BO3 | 26.00 | mg/l | 0.026 | 0.42 | |
| SrCl2 | 24.00 | mg/l | 0.024 | 0.151 | |
| NaF | 3.00 | mg/l | 0.003 | 0.071 | |
| 1 dissolve all components in water, 121℃ autocleave 20min, store in room temperature | |||||
Metadata
| Creator: | Taiyang Jin (Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences) |
| Author(s): | Taiyang Jin |
| Published: | 21.06.23 |
| Last modified: | 21.06.23 |
| Source: | MediaDive: public |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Filter |
| Edit in Medium builder | |