Main sol. 68
Internal Solution-ID: S108
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 4.0 | g | 4 | 29.393 | |
| K2HPO4 | 4.0 | g | 4 | 22.965 | |
| MgSO4 x 7 H2O | 0.8 | g | 0.8 | 3.246 | |
| NH4Cl | 0.4 | g | 0.4 | 7.478 | |
| Trace element solution (Vishniac & Santer, 1957) | 5.0 | ml | - | - | |
|
Bromocresol purple
(saturated aqueous solution) |
0.5 | ml | - | - | |
|
Agar
, for solid medium
(Bacto) optional |
15.0 | g | 15 | 44.599 | |
| Na2S2O3 x 5 H2O | 10.0 | g | 10 | 40.292 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except thiosulfate, dispense into suitable cultivation vessels and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution. Adjust pH of complete medium to 6.6 - 7.0. | |||||
Media with this solution
| 68 | HALOTHIOBACILLUS NEAPOLITANUS MEDIUM |