Main sol. 526
Internal Solution-ID: S1162
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 5-Aminovaleric acid | 15.00 | g | 15 | 128.041 | |
| K2HPO4 | 8.70 | g | 8.7 | 49.95 | |
| Yeast extract | 7.50 | g | 7.5 | - | |
| Trace elements solution VR | 4.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| NaHCO3 | 2.50 | g | 2.5 | 29.759 | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.25 | 1.423 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate and cysteine), adjust pH to 7.0 with NaOH and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min. Add and dissolve solid bicarbonate and cysteine, dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving adjust pH of medium to 7.6 - 7.8 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. | |||||
Media with this solution
| 526 | CLOSTRIDIUM VIRIDE MEDIUM |