Main sol. 692
Internal Solution-ID: S1440
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 15.0 | g | 14.677 | 251.148 | |
| NaHCO3 | 10.0 | g | 9.785 | 116.475 | |
| KH2PO4 | 0.3 | g | 0.294 | 2.157 | |
| NH4Cl | 0.5 | g | 0.489 | 9.146 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
|
NiCl2 x 6 H2O
(0.1% w/v) |
2.0 | ml | 0.002 | 0.015 | |
| Yeast extract | 0.5 | g | 0.489 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 4.9e-4 | 0.002 | |
| Na2CO3 | 4.0 | g | 3.914 | 36.928 | |
|
Methanol
(50% v/v) |
10.0 | ml | 7.75 | 241.855 | |
| Na2S x 9 H2O | 1.0 | g | 0.978 | 4.074 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, methanol and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and adjust pH to 9.0 - 9.2, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add methanol (50% v/v solution) and sulfide from anoxic stock solutions autoclaved under 100% N2 gas. Adjust pH of complete medium to 9.2 - 9.4. | |||||
Media with this solution
| 692 | METHANOSALSUM WeN5 MEDIUM |