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Main sol. 783

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Internal Solution-ID: S1588

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 7.00 g 7 28.4
MgCl2 x 6 H2O 5.50 g 5.5 27.053
KCl 0.65 g 0.65 8.719
NaBr 0.10 g 0.1 0.972
NaHCO3 2.00 g 2 23.808
NH4Cl 0.15 g 0.15 2.804
K2HPO4 0.15 g 0.15 0.861
CaCl2 x 2 H2O 0.50 g 0.5 3.401
Modified Wolin's mineral solution II 10.00 ml - -
Sulfur
(powdered) 		0.50 g 0.5 15.591
Distilled water 1000.00 ml - -
1 Prepare the medium aerobically and adjust pH to 7.0 using H2SO4. Fill 20 ml medium into 100 ml serum bottles under H2/CO2 (80/20) gas and seal with a rubber stopper. Steam medium for 3 hours on each of 3 successive days. Before use add 100 µl of a sterile CaCO3 solution (10%). Give an atmosphere of H2/CO2 with an overpressure of two atmospheres and 20 ml filter-sterilized air. Incubate under shaking at 60°C.
783 HYDROGENOTHERMUS HIRSCHII MEDIUM