Main sol. 829
Internal Solution-ID: S1679
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(SIGMA) |
30.00 | g | 29.851 | - | |
| NH4Cl | 1.00 | g | 0.995 | 18.602 | |
| KH2PO4 | 0.35 | g | 0.348 | 2.559 | |
| MES [2-(N-morpholino) ethane sulfonic acid] | 1.95 | g | 1.94 | 9.938 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Sulfur , powdered | 10.00 | g | 9.95 | 310.267 | |
| Na2CO3 | 0.50 | g | 0.498 | 4.694 | |
| Seven vitamins solution | 1.00 | ml | - | - | |
| Growth-stimulating factors | 1.00 | ml | - | - | |
| Na-dithionite solution (5% w/v) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfur, carbonate, vitamins, growth-stimulating factors and Na-dithionite), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Adjust pH to 6.0 and dispense under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials which contain already the appropriate amount of sulfur, only to 30% of their volume to allow for a large head space. Autoclave at a temperature of 110°C for 20 min! Add vitamins and growth-stimulating factors from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate and Na-dithionite from sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins and Na-dithionite are sterilized by filtration. Adjust pH of the complete medium to 6.0, if necessary. | |||||
| 2 After inoculation use 1 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture. | |||||
Media with this solution
| 829 | DESULFUROBACTERIUM MEDIUM |