Back to all solutions
Na-dithionite solution (5% w/v)
Internal Solution-ID: S6262
This solution is used in 8 media and was first defined in medium 829.
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
NaOH
(1 N) |
0.5 | ml | - | - | |
| Na2S2O4 | 0.5 | g | 50 | 287.175 | |
| Distilled water | 9.5 | ml | - | - | |
| 1 Add 1 N NaOH to distilled water and make the solution anoxic by sparging with 100% N2 gas. Then dissolve the Na-dithionite and filter-sterilize the solution into anoxic Hungate tubes. The final pH of the solution should be between 9 and 10. Store the prepared solution in a dark and cool place. Prepare only small amounts of stock solution, as Na-dithionite decomposes rapidly. | |||||