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346. DESULFALLAS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 965.0 ml - -
2 Solution B 20.0 ml - -
3 Solution C 10.0 ml - -
4 Solution D 10.0 ml - -
5 Solution E 1.0 ml - -
6 Solution F 10.0 ml - -
7 Solution G 1.0 ml - -
8 Solution H 1.7 ml - -
Instructions:
  1. Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic. Thereafter, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions C, D, E, F, G and H are prepared separately under 100% N2 gas atmosphere. Filter sterilize solutions D, F and G. To complete the medium appropriate amounts of solutions B to H are added to the sterile solution A in the sequence as indicated. Adjust pH of the complete medium to 7.2 - 7.5, if necessary.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 3.106 21.864
2 0.20 g 0.207 1.521
3 0.30 g 0.311 5.806
4 0.15 g 0.155 1.056
5 0.40 g 0.414 2.037
6 1.00 g 1.035 17.713
7 Trace element solution SL-10 1.00 ml - -
8
(0.1% w/v)
0.50 ml 5.2e-4 0.002
9 965.00 ml 997.174 55351.5
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 g 50 595.191
2 20 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 ml 71.818 1558.95
2 10 ml 907.461 50371.7
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.7 g 70 485.764
2 10.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Clarified rumen fluid 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 25 mg 25 -
2 1 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 50.0 mg 25 104.088
2 1.7 ml 848.476 47097.5
Solution occurs in 32 other media (first defined in medium 1310 )
Equipment needed: Centrifuge Filter Autoclave
Instructions:
  1. Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 minand then centrifuged at 27,000 g for 20 min The supernatant is made anoxic by sparging with 100% N2 gas for 15 min dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at - 20°C.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Autoclave, Centrifuge, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 995.784 55274.3
Na2SO4 2.941 20.705
NaHCO3 0.981 11.682
NaCl 0.98 16.774
Ethanol 0.705 15.299
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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