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829b. THERMODESULFOBACTERIUM HYDROGENIPHILUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(SIGMA)
30.00 g 29.881 -
2 1.00 g 0.996 18.62
3 0.35 g 0.349 2.562
4 1.95 g 1.942 9.948
5 Trace element solution SL-10 1.00 ml - -
6 Selenite-tungstate solution 1.00 ml - -
7
(0.1% w/v)
0.50 ml 5.0e-4 0.002
8 0.50 g 0.498 4.699
9 0.50 g 0.498 -
10 Seven vitamins solution 1.00 ml - -
11 Growth-stimulating factors 1.00 ml - -
12 20.00 mg 0.02 -
13 1000.00 ml 994.23 55188.1
Instructions:
  1. Dissolve ingredients (except carbonate, yeast extract, vitamins, growth-stimulating factors and dithionite), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Adjust pH to 6.0 and dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, vitamins, growth-stimulating factors and dithionite from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins and dithionite are sterilized by filtration. Adjust pH of the complete medium to 6.5, if necessary. After inoculation use 2 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 1 other media (first defined in medium 829 )
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 g 5 56.75
2 5 g 5 48.956
3 5 g 5 48.956
4 5 g 5 48.956
5 2 g 2 17.218
6 6 g 6 50.81
7 1000 ml 998.207 55408.8
Instructions:
  1. Dissolve ingredients, adjust pH to 9.0 with NaOH, then autoclave under 100% N2 gas.
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % H2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.3
Sea Salt 29.881 -
2-(N-Morpholino)ethanesulfonic acid 1.942 9.948
NH4Cl 0.996 18.62
Na2CO3 0.498 4.699
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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