Main sol. 993
Internal Solution-ID: S2024
This solution is used in 4 media and was first defined in medium 993.
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
L-Asparagine
(anhydrous basis) |
1 | g | 1 | 7.569 | |
| Glycerol | 10 | g | 10 | 108.589 | |
|
K2HPO4
(anhydrous basis) |
1 | g | 1 | 5.741 | |
| Distilled water | 1000 | ml | - | - | |
| Trace element solution | 1 | ml | - | - | |
| Agar | 20 | g | 20 | 59.465 | |
| 1 The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes. | |||||
| 2 Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish. | |||||