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993. ISP 5 MEDIUM

Culture medium recipe

Vol.
×

M 993 #

ml
Solution occurs in 3 other media (first defined in medium 993 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(anhydrous basis)
1 g 0.999 7.561
2 10 g 9.99 108.476
3
(anhydrous basis)
1 g 0.999 5.736
4 1000 ml 997.21 55353.5
5 Trace element solution 1 ml - -
6 20 g 19.98 -
Instructions:
  1. The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15- 20 minutes.
  2. Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.
Solution occurs in 8 other media (first defined in medium 84 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.1 g 1 3.597
2 0.1 g 1 5.053
3 0.1 g 1 3.478
4 100.0 ml 998.207 55408.8
Instructions:
  1. pH 7,2

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: unknown

Associated strains

All strains for this medium: 10
Strains with modifications: 7
993 ISP 5 MEDIUM
a NESTERENKONIA MODIFIED MEDIUM
b MODIFIED ISP5 MEDIUM
c MODIFIED ISP5 MEDIUM WITH SODIUM CHLORIDE

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
Agar 19.98 -
Glycerol 9.99 108.476
L-Asparagine 0.999 7.561
K2HPO4 0.999 5.736
MnCl2 x 4 H2O 1.0e-3 0.005
FeSO4 x 7 H2O 1.0e-3 0.004
ZnSO4 x 7 H2O 1.0e-3 0.003

Cultivation metadata from 10 strains BacDive

halophily extreme 1 strain
halophily not 1 strain
halophily slightly 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 2 10 strains
oxygen aerobe 1 strain
temperature mesophilic 10 strains

Similar media

J60. GLYCEROL-ASPARAGINE AGAR (ISP-5) 82 %
J63. SAUTON'S SYNTHETIC MEDIUM 77 %
547. ISP MEDIUM 4 75 %
J62. GLYCEROL-CALCIUM MALATE AGAR 74 %
J610. SPHAEROTILUS MEDIUM 72 %

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