993c
993c .
MODIFIED ISP5 MEDIUM WITH SODIUM CHLORIDE
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 993c #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 993 | 1000 | ml | - | - | |
| NaCl | 10 | % | 100 | 1711.16 | |
| 1 Prepare medium 993 supplemented with NaCl (10 %). pH should be 7.2. | |||||
| 2 Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish. | |||||
Solution occurs in 3
other media (first defined in Medium 993).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
L-Asparagine
(anhydrous basis) |
1 | g | 1 | 7.569 | |
| Glycerol | 10 | g | 10 | 108.589 | |
|
K2HPO4
(anhydrous basis) |
1 | g | 1 | 5.741 | |
| Distilled water | 1000 | ml | - | - | |
| Trace element solution | 1 | ml | - | - | |
| Agar | 20 | g | 20 | 59.465 | |
| 1 The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes. | |||||
| 2 Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeSO4 x 7 H2O | 0.1 | g | 1 | 3.597 |
| MnCl2 x 4 H2O | 0.1 | g | 1 | 5.053 |
| ZnSO4 x 7 H2O | 0.1 | g | 1 | 3.478 |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 25.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 4 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 100 | 1711.16 |
| Agar | 20 | 59.465 |
| Glycerol | 10 | 108.589 |
| L-Asparagine | 1 | 7.569 |
| K2HPO4 | 1 | 5.741 |
| Show more | ||
