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Main sol. 1082

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Internal Solution-ID: S2191

Equipment needed: Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 0.297 2.183
K2HPO4 0.3 g 0.297 1.705
NH4Cl 1.0 g 0.99 18.51
NaCl 10.0 g 9.901 169.421
KCl 0.1 g 0.099 1.328
CaCl2 x 2 H2O 0.1 g 0.099 0.673
MgCl2 x 6 H2O 0.5 g 0.495 2.435
Modified Wolin's mineral solution 10.0 ml - -
Trypticase peptone
(BD BBL) 		10.0 g 9.901 -
Yeast extract 2.0 g 1.98 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 0.002
L-Cysteine HCl x H2O 0.5 g 0.495 2.819
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except cysteine), then sparge medium for 30 - 45 min with 100% N2 gas atmosphere to make it anoxic. Add cysteine, adjust pH to 7.1 and dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. The pH of the complete medium should be 7.0 - 7.2.
1082 AMINIPHILUS MEDIUM