Main sol. 1100
Internal Solution-ID: S2216
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 3.00 | g | 2.947 | 20.747 | |
| KH2PO4 | 0.20 | g | 0.196 | 1.444 | |
| NH4Cl | 0.30 | g | 0.295 | 5.509 | |
| NaCl | 7.00 | g | 6.876 | 117.663 | |
| Trace element solution SL-11 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| MgCl2 x 6 H2O | 3.10 | g | 3.045 | 14.979 | |
| KCl | 0.50 | g | 0.491 | 6.588 | |
| CaCl2 x 2 H2O | 0.15 | ml | - | - | |
| Yeast extract | 0.50 | g | 0.491 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
| NaHCO3 | 1.50 | g | 1.473 | 17.54 | |
| Na-L-lactate | 2.00 | g | 1.965 | - | |
| Neutralized sulfide solution 3% (w/v) | 15.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, lactate and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add lactate and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 7.2, if necessary. | |||||
Media with this solution
| 1100 | DESULFACINUM MEDIUM |