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Main sol. 1245

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Internal Solution-ID: S2474

Compound Amount Unit Conc. [g/L] Conc. [mM]
Proteose peptone no. 2 5.0 g 5 -
Heart Infusion Broth 5.0 g 5 -
Yeast extract 5.0 g 5 -
Potassium Phosphate , dibasic 2.0 g 2 14.697
Sodium Chloride 5.0 g 5 85.558
Magnesium sulfate 0.1 g 0.1 0.831
Acetic acid 1.6 ml - -
Propionic acid 0.6 ml - -
n-Butyric acid 0.4 ml - -
n-Valeric acid 0.1 ml - -
Isovaleric acid 0.1 ml - -
Isobutyric acid 0.1 ml - -
2-Methylbutyric acid 0.1 ml - -
Vitamin K1
(1% soln) 		0.1 ml 1.0e-3 0.002
L-Cysteine 0.5 g 0.5 4.127
Fetal bovine serum , inactivated 100.0 ml - -
Sugar Stock Solution 100.0 ml - -
Distilled water 800.0 ml - -
1 OTEB-medium is available from Anaerobe Systems (http://www.anaerobesystems.com)
2 The following text was taken from the above page: - ORAL TREPONEME ENRICHMENT BROTH (OTEB) - Products: AS-603 Oral Treponeme Enrichment Broth (OTEB) 10 tubes / pkg
3 Intended Use: OTEB is an enriched medium for the isolation, cultivation and maintenance of Treponemes found as part of the oral flora.
4 OTEB is a complex peptone-yeast extract medium containing volatile fatty acids and serum for a source of long chain fatty acids. Volatile fatty acids and serum are necessary nutrients for recovery of Treponemes from the oral flora. Inoculation of the medium can be done using a gassing cannula or inside an anaerobic chamber. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.
5 Final pH 7.0 +/- 0.2 at 25 degrees C.
6 Final volume 7.0 ml +/- 0.7.
1245 OTEB-MEDIUM