Solution 1
Internal Solution-ID: S2973
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Hutner's salts | 20 | ml | - | - | |
|
Tris-HCl buffer
(0.1M, pH 7.5) |
50 | ml | - | 5 | |
| Artifical sea water | 500 | ml | - | - | |
| Agar | 18 | g | 18.947 | 56.336 | |
| Distilled water | 380 | ml | - | - | |
| 1 Adjust pH to 7.5, autoclave at 121°C for 20 min. | |||||
| 2 For agar plates increase the pH of the Tris/HCl buffer to 8.5. | |||||
Media with this solution
| 1444 | PIRELLULA MEDIUM (M30a) |