Main sol. 1478
Internal Solution-ID: S3026
Equipment needed: Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 72.000 | g | 72 | 1232.03 | |
| K2HPO4 | 2.500 | g | 2.5 | 14.353 | |
| (NH4)2SO4 | 0.125 | g | 0.125 | 0.946 | |
| Na2CO3 | 142.500 | g | 142.5 | 1344.49 | |
| NaHCO3 | 22.500 | g | 22.5 | 267.835 | |
| Trace element solution | 1.000 | ml | - | - | |
| Sulfur powder | 2.000 | g | 2 | 7.797 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Prepare the liquid medium. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Steam medium for 3h on each of 3 successive days. Before inoculation add from sterile stock solutions: | |||||
| MgCl2 x 6 H2O | 1.200 | mM | 0.244 | 1.2 | |
| NaHCO3 | 1.250 | mM | 0.105 | 1.25 | |
| NH4Cl | 3.000 | mM | 0.16 | 3 | |
| Sodium formate | 50.000 | mM | 3.4 | 50 | |
| Sodium acetate | 2.000 | mM | 0.164 | 2 | |
| Yeast extract | 20.000 | mg/l | 0.02 | - | |
| Vitamine mixture | 1.000 | ml | - | - | |
|
Disodium sulfide
(1M filter sterilized stock) |
1.000 | mM | - | 1 | |
Media with this solution
| 1478 | NATRANAEROARCHAEUM MEDIUM AARC1 |