Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Bacto peptone | 3.00 | g | 3 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| Betaine | 1.25 | g | 1.25 | 10.67 | |
| Sodium pyruvate | 1.25 | g | 1.25 | 11.36 | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
| Wolin's vitamin solution | 10.00 | ml | - | - | |
| Glycerol | 10.00 | ml | - | - | |
| Distilled water | 970.00 | ml | - | - | |
| 1 Adjust to pH 7.2 | |||||
Media with this solution
| 1484 | Glycerol-Yeast extract-Peptone Medium |