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Main sol. 1487

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Internal Solution-ID: S3049

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 16.50 g 16.321 279.269
MgCl2 x 6 H2O 3.50 g 3.462 17.028
Na2SO4 2.75 g 2.72 19.15
KCl 0.50 g 0.495 6.634
NaNO3 0.50 g 0.495 5.819
CaCl2 x 2 H2O 0.40 g 0.396 2.691
NH4Cl 0.25 g 0.247 4.623
Trace element solution 10.00 ml - -
Na-acetate 0.25 g 0.247 3.014
Disodium succinate 0.50 g 0.495 3.052
Casamino acids
(BD Bacto) 		0.50 g 0.495 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
K2HPO4 0.15 g 0.148 0.852
Na2CO3 1.50 g 1.484 13.999
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.25 g 0.247 1.408
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except hydrogenphosphate, carbonate, vitamins and cysteine), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add hydrogenphosphate, vitamins and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The vitamin solution should be sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2, if necessary.
1487 MAGNETOVIBRIO MEDIUM