Main sol. 1524
Internal Solution-ID: S3151
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mineral salt solution | 20.00 | ml | - | - | |
|
Peptone
(Bacto) |
0.50 | g | 0.336 | - | |
| Glucose | 0.25 | g | 0.168 | 0.931 | |
|
HEPES
(10mM) |
2.38 | g | 1.597 | 6.703 | |
| Artifical sea water | 500.00 | ml | - | - | |
|
Agar
(Bacto) |
15.00 | g | 10.067 | 29.932 | |
| Distilled water | 475.00 | ml | - | - | |
| 1 Sterilize 20 min/121°C. After cooling to 60°C add to the medium: | |||||
| Vitamin solution (double conc.) | 5.00 | ml | - | - | |
| 2 Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH). | |||||
Media with this solution
| 1524 | PLANCTOMYCETES MEDIUM NH11 |