Main sol. 1586
Internal Solution-ID: S3277
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract
(Difco) |
4 | g | 4 | - | |
Malt extract
(Difco) |
10 | g | 10 | - | |
Dextrose
(Difco) |
4 | g | 4 | 20.185 | |
Trace element solution SL-10 | 1 | ml | - | - | |
Agar | 20 | g | 20 | 59.465 | |
Distilled water | 1000 | ml | - | - | |
1 pH 7.2 | |||||
2 Sterilize 20 min/121°C. After cooling to 60°C add to the medium: | |||||
Vitamin solution | 5 | ml | - | - |
1586 | ISP2 MODIFIED MEDIUM FOR METHYLIBIUM |