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Main sol. J335

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Internal Solution-ID: S4053

Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.78 g 0.765 4.39
KH2PO4 0.75 g 0.735 5.403
Na3-EDTA 0.04 g 0.039 -
FeSO4 x H2O 0.01 g 0.01 0.058
MgSO4 x H2O 0.25 g 0.245 2.036
CaCl2 x H2O 0.03 g 0.029 0.265
NaCl 0.20 g 0.196 3.355
NH4Cl 27.00 mg 0.026 0.495
Na2SO4 2.80 g 2.745 -
Sodium acetate 0.16 g 0.157 1.912
Trace vitamins 10.00 ml - -
Trace element solution 10.00 ml - -
L-Cysteine HCl x H2O 0.25 g 0.245 -
Distilled water 1000.00 ml - -
1 Adjust pH to 5.5. Fill the gas phase with H2-CO2 (80:20).
J335 JCM MEDIUM No. 335